FIG. 3.

Lung chemokine gene expression at early time points after RSV challenge. Groups of three to four mice were immunized three times i.m. with 50 μg of BBG2Na (●), FI-RSV (stock 1 diluted at 1/250) (■), or PBS (▴) in presence of Alhydrogel. Mice were sacrificed 3, 6, 9, 12, and 24 h after the i.n. challenge with RSV. Their lungs were removed in order to analyze chemokine gene expression by a semiquantitative RT-PCR-ELISA method. Naive unchallenged mice were used to determine the baseline level for each chemokine (horizontal line or x axis if not visible). Results shown are means ± SD (error bars) of OD₄₀₅ for the chemokine mRNA relative to the OD₄₀₅ for the β-actin mRNA.