FIG. 4.

Lung chemokine gene expression at later time points after RSV challenge. Groups of three to seven mice were immunized three times i.m. with FI-RSV (stock 1 diluted at 1/250) (grey bars), PBS (white bars), or 20 μg of BBG2Na (black bars), in the presence of Alhydrogel. Mice were sacrificed 5 and 7 days after the intranasal challenge with RSV. Their lungs were removed for a semiquantitative RT-PCR-ELISA analysis of chemokine gene expression. Naive unchallenged mice were used to determine the baseline level for each chemokine (horizontal lines or x axis if not visible). Results shown are means ± SD (error bars) of OD₄₀₅ for the chemokine mRNA relative to the OD₄₀₅ for the β-actin mRNA. ∗, P < 0.05 (calculated by the Kolmogorov-Smirnov test).