Fig 1. fshr-1 is required for neuromuscular function in multiple assays.

(A) Aldicarb paralysis assays, (B) swimming assays, and (C) multi-worm tracking assays were performed on wild type worms, fshr-1(ok778) mutants, rescued animals (Rescue) re-expressing fshr-1 under the endogenous fshr-1 promoter (Pfshr-1, ibtEx15) in the fshr-1 mutant background, and over-expression (OE) animals expressing fshr-1 under the endogenous fshr-1 promoter (Pfshr-1, ibtEx15). (A) (Left panel) Representative aldicarb assays showing the mean percentage of worms paralyzed on 1mM aldicarb ± s.e.m. for n = 3 plates of approximately 20 young adult animals each per strain. (Right panels) Bar graphs showing cumulative mean data ± s.e.m. pooled from 3–4 independent experiments for worms paralyzed at the timepoint indicated by an asterisk (*) in the left panel. Scatter points show individual plate averages. Statistical significance of the data was analyzed using a one-way ANOVA and Tukey’s post hoc test or a Wilcoxon Rank Sum test followed by a Steel-Dwass multiple comparison analysis, as appropriate. (B) Mean body bends per minute ± S.D. obtained in swimming experiments. Each scatter point represents an independent experiment testing 30 animals per genotype. (C) Mean body bend amplitude ± S.D. obtained from multi-worm tracking experiments. Each scatter point represents an independent experiment from a population average of 20 animals. Statistical significance of the data was analyzed using a one-way ANOVA with Tukey’s multiple comparison. For A-C, results of analyses for which p ≤ 0.05 are indicated by horizontal lines above the bars. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, **** p ≤ 0.0001, n.s., not significant.