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. 2024 Nov 19;20(11):e1011461. doi: 10.1371/journal.pgen.1011461

Fig 7. Regulation of neuromuscular function by intestinal FSHR-1 requires intestinally expressed GSA-1, ACY-1, AND SPHK-1, as well as the GLPA-1 and GPLB glycoproteins.

Fig 7

(A-C) Mean body bends per minute ± S.D. obtained in swimming experiments testing intestinal RNAi sensitized [Pnhx-2::rde-1; rde-1(ne219)] control-treated animals overexpressing fshr-1 in the intestine (Pges-1, ibtEx35, Intestinal fshr-1 OE), treated with feeding RNAi targeting (A) gsa-1, (B) acy-1, or (C) sphk-1, or animals with intestinal FSHR-1 overexpression and gsa-1, acy-1, or sphk-1 RNAi compared to L4440 empty vector-treated worms (Control). Each scatter point represents an independent experiment testing 10 animals per genotype. (D-F) Mean body bends per minute ± S.D. obtained in swimming experiments testing animals overexpressing fshr-1 in the intestine (Pges-1, ibtEx35), (D) gpla-1(ibt1), (E) gplb-1(itb4), or (F) gpla-1gplb-1 mutations compared to animals with both intestinal fshr-1 overexpression and glycoprotein mutation, and wild type controls. Each scatter point represents an independent experiment testing 30 animals per genotype. For all experiments, one-way ANOVA and Tukey’s post hoc tests were used to compare the means of the datasets (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001; n.s., not significant).