Table 1. C. elegans strains used in this study.
(*) indicates number of backcrosses to N2.
| Strain Number | Genotype | Reference |
|---|---|---|
| N2 | N2 | |
| RB911 | fshr-1(ok778) | C. elegans Deletion Mutant Consortium [123] |
| JRK165 | fshr-1(ok778)*6 | This study |
| KG522 | acy-1(md1756) | Cho et al (2007) [27] |
| WY371 | acy-1(md1756); fshr-1(ok778) | Cho et al (2007) [27] |
| KG421 | gsa-1(ce81) | Cho et al (2007) [27] |
| WY415 | gsa-1(ce81); fshr-1(ok778) | Cho et al (2007) [27] |
| VC916 | sphk-1(ok1097) | C. elegans Deletion Mutant Consortium [123] |
| JRK166 | sphk-1(ok1097)*4 | This study |
| JRK168 | sphk-1(ok1097)*4; fshr-1(ok778)*6 | This study |
| KP3814 | nuIs152 (Punc-129::SNB- 1::GFP)*2 | Sieburth et al (2005) [27] |
| JRK42 | nuIs152; fshr-1(ok778)*3 | This study |
| KP3928 | nuIs165 (Punc-129::UNC-10::GFP) | Sieburth et al (2005) [27] |
| JRK76 | nuIs165; fshr-1(ok778) | This study |
| KP3091 | nuIs159 (Punc-129::SYD-2::GFP)*10, | Sieburth et al (2005) [27] |
| JRK67 | nuIs159; fshr-1 | This study |
| TV18676 | wyIs687 (Punc-17::CLA-1::GFP) | P. Kurshan |
| JRK124 | wyIs687; fshr-1 | This study |
| KP3894 | nuIs195 (Punc-129::ins-22::VENUS) | Sieburth et al (2005) [50] |
| JRK96 | nuIs195; fshr-1(ok778) | This study |
| MT20435 | nIs463 [Punc-17::snb-1::SEP(SpH)] | Paquin et al (2016) [65] |
| JRK164 | nIs463; fshr-1(ok778)*3 | This study |
| JKR181 | nIs463; unc-13 (s69) | This study |
| IBE89 | ibtEx15 [fshr-1p::fshr-1gDNA::sl2::mKate 10 ng/μl; unc-122p::gfp 25 ng/μl]; fshr-1(ok778) | Kenis et al (2023) [48] |
| IBE225 | ibtEx35 [ges-1p::fshr-1 cDNA::sl2::GFP 30 ng/μl; myo-2p::mCherry 10 ng/μl]; fshr-1(ok778) | Kenis et al (2023) [48] |
| IBE333 | ibtEx51 [mir-228p::fshr-1 cDNA::sl2::mKate 20 ng/μl; unc-122p::GFP 10 ng/μl]; fshr-1(ok778) | Kenis et al (2023) [48] |
| IBE223 | ibtEx34[rab-3p::fshr-1 cDNA::sl2::mKate 20 ng/μl; unc-122p::gfp 25 ng/μl]; fshr-1 (ok778) | Kenis et al (2023) [48] |
| IBE465 | ibtEx67[rgef-1p::fshr-1 cDNA::sl2::GFP::tbb-2 3’UTR 40 ng/μl; myo-2p::mCherry 10 ng/μl]; fshr-1 (ok778) | Kenis et al (2023) [48] |
| IBE88 | gpla-1(ibt1)*4 | Kenis et al (2023) [48] |
| JRK183 | ibtEx15 [fshr-1p::fshr-1gDNA::sl2::mKate 10 ng/μl; unc-122p::gfp 25 ng/μl]—outcrossed from IBE89 | This study |
| JRK94 | agEx43 [Pfshr-1:: fshr-1; Pmyo-2:: NLS:: cherry]; fshr-1(ok778); nuIs152 | This study |
| JRK167 | ibtEx35 [ges-1p::fshr-1 cDNA::sl2::GFP 30 ng/μl; myo-2p::mCherry 10 ng/μl]; fshr-1(ok778); nuIs152 | This study |
| JRK177 | ibtEx35 [ges-1p::fshr-1 cDNA::sl2::GFP 30 ng/μl; myo-2p::mCherry 10 ng/μl]—outcrossed from IBE225 | This study |
| JRK182 | ibtEx51 [mir228p::fshr-1 cDNA::sl2::mKate 20 ng/μl; unc-122p::GFP 10 ng/μl]—outcrossed from IBE333 | This study |
| IBE208 | gplb-1(ibt4)*4 | Kenis et al (2023) [48] |
| IBE149 | gpla-1(ibt1) V; fshr-1(ok778) V | Kenis et al (2023) [48] |
| IBE420 | gplb-1 (ibt4) V; fshr-1(ok778) V | Kenis et al (2023) [48] |
| IB265 | gpla-1(ibt1) V; gplb-1(ibt4) V | Kenis et al (2023) [48] |
| IB421 | gpla-1(ibt1) V; gplb-1 (ibt4) V; fshr-1(ok778) V | Kenis et al (2023) [48] |
| VP303 | kbIs7;rde-1(ne219) | Espelt et al (2005) [84] |
| JRK186 | ibtEx35; kbIs7;rde-1(ne219) | This study |
| JRK189 | ibtEx67[rgef-1p::fshr-1 cDNA::sl2::GFP::tbb-2 3’UTR 40 ng/μl; myo-2p::mCherry 10 ng/μl]—outcrossed from IBE465 | This study |
| JRK192 | ibtEx35; gpla-1(ibt1)*6 | This study |
| JRK197 | ibtEx35; gplb-1(ibt4)*4 | This study |
| JRK199 | ibtEx35; gpla-1(ibt1)*6; gplb-1(ibt4)*4 | This study |