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. 2024 Dec 3;19(12):e0314516. doi: 10.1371/journal.pone.0314516

Table 3. Sensitivity and specificity of the in-house iELISA.

Test RFFIT Total Sensitivity (%) Specificity (%) Kappa value
Positive Negative
In-house ELISA Positive 190 7 197 90.48 80.00 0.647
Negative 20 28 48
Total 210 35 245

Note: Kappa (κ) value of 0.61–0.80 is considered as a substantial agreement between the two tests [29].