Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Dec 3;12:RP87120. doi: 10.7554/eLife.87120

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023, Drougard et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

PMC Copyright notice

Figure 4. — (A) BODIPY staining on primary microglia challenged for 24 hr with BSA or palmitate and the lipid droplets quantification graph (n=20). (B) Schematic depicting the timeline for the tracing experiments (¹³C-palmitate or ¹³C-glucose) on primary microglial challenged for 24 hr with BSA or palmitate. (C) ¹³C-palmitate incorporation into palmitoylcarnitine (m+16) and acetylcarnitine (m+2) after 4 hr tracing experiment on primary microglia pretreated for 24 hr with BSA or palmitate (n=3). (D) ¹³C-palmitate incorporation into acetyl serine (m+2) after 4 hr tracing experiment on primary microglia pretreated for 24 hr with BSA or palmitate (n=3). (E) ¹³C-palmitate incorporation into aconitate, alpha-ketoglutarate, fumarate, malate (m+2) after 4 hr tracing experiment on primary microglia pretreated for 24 hr with BSA or palmitate (n=3). (F) ¹³C-palmitate incorporation into glutamate (m+2) after 4 hr tracing experiment on primary microglia pretreated for 24 hr with BSA or palmitate (n=3). (G) ¹³C-palmitate incorporation into glutamate (m+2) released during the 4 hr tracing experiment by primary microglia pretreated for 24 hr with BSA or palmitate (n=3). The results are graphed in relative abundance. (H) ¹³C-palmitate incorporation into itaconate (m+1) released during the 4 hr tracing experiment by primary microglia pretreated for 24 hr with BSA or palmitate (n=3). The results are graphed in relative abundance. (I) ¹³C-palmitate incorporation into succinate (m+2) released during the 4 hr tracing experiment by primary microglia pretreated for 24 hr with BSA or palmitate (n=3). The results are graphed in relative abundance. (J) ¹³C-glucose incorporation into the intracellular glucose pool (m+6) after 6 hr tracing experiment on primary microglia pretreated for 24 hr with BSA or palmitate (n=3). (K) ¹³C-glucose incorporation into glutamate (m+2) released during the 6 hr tracing experiment by primary microglia pretreated for 24 hr with BSA or palmitate (n=3). The results are graphed in relative abundance. (L) ¹³C-glucose incorporation into itaconate (m+1) released during the 6 hr tracing experiment by primary microglia pretreated for 24 hr with BSA or palmitate (n=3). The results are graphed in relative abundance. (M) ¹³C-glucose incorporation into succinate (m+2) released during the 6 hr tracing experiment by primary microglia pretreated for 24 hr with BSA or palmitate (n=3). The results are graphed in relative abundance. (N) ¹³C-glucose incorporation into lactate (m+3) released during the 6 hr tracing experiment by primary microglia pretreated for 24 hr with BSA or palmitate (n=3). The results are graphed in relative abundance. (O) Schematic depicting the metabolic pathways used by the primary microglial challenged for 24 hr with BSA (black arrow) or palmitate (red arrow) during the different tracing (¹³C-palmitate or ¹³C-glucose). Data are presented as mean ± SEM. *p<0.05, **p<0.01, ***p<0.001 as determined by two-tailed Student’s t-test and two-way ANOVA followed by Bonferroni post hoc test.

Figure 4—figure supplement 1. — (A) BODIPY staining on primary microglia challenged for 24 hr with BSA or palmitate and the lipid droplets quantification graph (n=20). (B) Schematic depicting the timeline for the tracing experiments (¹³C-palmitate or ¹³C-glucose) on primary microglial challenged for 24 hr with BSA or palmitate. (C) ¹³C-palmitate incorporation into palmitoylcarnitine (m+16) and acetylcarnitine (m+2) after 4 hr tracing experiment on primary microglia pretreated for 24 hr with BSA or palmitate (n=3). (D) ¹³C-palmitate incorporation into acetyl serine (m+2) after 4 hr tracing experiment on primary microglia pretreated for 24 hr with BSA or palmitate (n=3). (E) ¹³C-palmitate incorporation into aconitate, alpha-ketoglutarate, fumarate, malate (m+2) after 4 hr tracing experiment on primary microglia pretreated for 24 hr with BSA or palmitate (n=3). (F) ¹³C-palmitate incorporation into glutamate (m+2) after 4 hr tracing experiment on primary microglia pretreated for 24 hr with BSA or palmitate (n=3). (G) ¹³C-palmitate incorporation into glutamate (m+2) released during the 4 hr tracing experiment by primary microglia pretreated for 24 hr with BSA or palmitate (n=3). The results are graphed in relative abundance. (H) ¹³C-palmitate incorporation into itaconate (m+1) released during the 4 hr tracing experiment by primary microglia pretreated for 24 hr with BSA or palmitate (n=3). The results are graphed in relative abundance. (I) ¹³C-palmitate incorporation into succinate (m+2) released during the 4 hr tracing experiment by primary microglia pretreated for 24 hr with BSA or palmitate (n=3). The results are graphed in relative abundance. (J) ¹³C-glucose incorporation into the intracellular glucose pool (m+6) after 6 hr tracing experiment on primary microglia pretreated for 24 hr with BSA or palmitate (n=3). (K) ¹³C-glucose incorporation into glutamate (m+2) released during the 6 hr tracing experiment by primary microglia pretreated for 24 hr with BSA or palmitate (n=3). The results are graphed in relative abundance. (L) ¹³C-glucose incorporation into itaconate (m+1) released during the 6 hr tracing experiment by primary microglia pretreated for 24 hr with BSA or palmitate (n=3). The results are graphed in relative abundance. (M) ¹³C-glucose incorporation into succinate (m+2) released during the 6 hr tracing experiment by primary microglia pretreated for 24 hr with BSA or palmitate (n=3). The results are graphed in relative abundance. (N) ¹³C-glucose incorporation into lactate (m+3) released during the 6 hr tracing experiment by primary microglia pretreated for 24 hr with BSA or palmitate (n=3). The results are graphed in relative abundance. (O) Schematic depicting the metabolic pathways used by the primary microglial challenged for 24 hr with BSA (black arrow) or palmitate (red arrow) during the different tracing (¹³C-palmitate or ¹³C-glucose). Data are presented as mean ± SEM. *p<0.05, **p<0.01, ***p<0.001 as determined by two-tailed Student’s t-test and two-way ANOVA followed by Bonferroni post hoc test.