Table 2. Metabolites released in media from BSA- or palmitate (PA)-treated neurons.
| Metabolite | N | Overall, N=12* | BSA, N=6* | PA, N=6* | p-Value† | q-Value ‡ |
|---|---|---|---|---|---|---|
| b_DL.Lactic.Acid | 12 | 7,768,093,932.0 (566,966,988.5) | 7,315,358,105.8 (237,880,097.3) | 8,220,829,758.2 (398,345,524.3) | 0.002 | 0.064 |
| b_D..Glutamine | 12 | 92,514,878.8 (9,477,866.2) | 99,910,567.8 (6,363,882.4) | 85,119,189.7 (5,084,875.9) | 0.002 | 0.064 |
| a_Acetyl.L.carnitine | 12 | 21,534,366.5 (5,136,659.2) | 25,486,005.2 (4,313,399.0) | 17,582,727.8 (1,401,821.0) | 0.004 | 0.085 |
| b_L.Serine | 12 | 8,927,932.4 (1,999,447.4) | 10,439,581.3 (1,620,380.1) | 7,416,283.5 (827,833.2) | 0.009 | 0.13 |
| b_X4.Oxoproline | 12 | 5,158,697.7 (2,093,379.7) | 6,584,223.3 (1,496,000.9) | 3,733,172.0 (1,589,279.7) | 0.026 | 0.3 |
| b_X3.Hydroxy.2.methyl.4.pyrone.tent. | 12 | 112,324,855.5 (18,985,842.0) | 122,558,080.8 (13,194,171.3) | 102,091,630.2 (19,173,036.1) | 0.065 | 0.6 |
| a_Adipic.acid.tent. | 12 | 463,554.3 (1,036,466.7) | 0.0 (0.0) | 927,108.7 (1,359,286.8) | 0.074 | 0.6 |
| a_D..Pyroglutamic.Acid | 12 | 287,577,957.7 (32,240,216.1) | 302,927,531.8 (25,661,286.0) | 272,228,383.5 (32,600,385.7) | 0.093 | 0.6 |
| b_L.Arabinose.or.isomer. | 12 | 427,996,608.9 (18,980,682.8) | 437,653,974.3 (18,029,045.8) | 418,339,243.5 (15,611,025.3) | 0.093 | 0.6 |
| b_L.Tyrosine | 12 | 85,928,933.3 (296,055,668.3) | 345,898.5 (293,093.1) | 171,511,968.0 (418,627,435.0) | 0.13 | 0.7 |
| b_Sodium.lauryl.sulfate | 12 | 22,598.3 (76,220.7) | 0.0 (0.0) | 45,196.5 (107,496.4) | 0.2 | 0.7 |
| b_Tridecanoic.acid | 12 | 113,734.1 (302,240.1) | 0.0 (0.0) | 227,468.2 (412,217.0) | 0.2 | 0.7 |
| a_Leucine | 12 | 27,980,174.9 (7,729,502.5) | 30,862,636.3 (2,851,217.9) | 25,097,713.5 (10,167,075.1) | 0.2 | 0.7 |
| b_Glutaric.acid.tent. | 12 | 440,497,616.4 (19,061,443.4) | 448,877,445.2 (22,010,672.2) | 432,117,787.7 (12,097,256.5) | 0.2 | 0.7 |
| a_PEG.n5.tent. | 12 | 344,189.2 (776,660.6) | 4,541.3 (11,123.9) | 683,837.0 (1,024,721.6) | 0.2 | 0.7 |
| a_L.Serine | 12 | 390,966.4 (346,737.4) | 527,905.0 (409,596.4) | 254,027.8 (227,430.6) | 0.2 | 0.7 |
| b_D..Glucose.or.isomer. | 12 | 5,354,625,351.9 (372,775,074.5) | 5,219,148,234.7 (282,140,339.8) | 5,490,102,469.2 (426,687,091.4) | 0.2 | 0.7 |
| b_AICA.ribonucleotide | 12 | 64,660,392.8 (5,188,450.2) | 63,405,196.7 (3,409,871.4) | 65,915,588.8 (6,619,333.0) | 0.2 | 0.7 |
| b_neuraminic.acid.tent. | 12 | 23,066,967.0 (2,864,562.7) | 24,475,099.5 (3,412,576.8) | 21,658,834.5 (1,283,781.1) | 0.2 | 0.7 |
| a_Betaine | 12 | 3,612,697.5 (2,636,694.6) | 2,762,143.5 (2,827,681.0) | 4,463,251.5 (2,358,539.4) | 0.3 | >0.9 |
| a_DL.Arginine | 12 | 3,821,072.9 (7,222,808.1) | 2,199,664.0 (5,388,054.4) | 5,442,481.8 (8,912,410.1) | 0.3 | >0.9 |
| a_L.Threonine | 12 | 5,270,710.2 (5,478,082.2) | 6,513,108.5 (5,492,782.8) | 4,028,311.8 (5,669,696.2) | 0.4 | >0.9 |
| a_Pantothenic.acid | 12 | 22,359,057.0 (26,015,488.1) | 28,020,428.8 (25,119,049.5) | 16,697,685.2 (27,947,862.2) | 0.4 | >0.9 |
| b_L.Leucine | 12 | 6,646,608.2 (3,705,017.3) | 7,399,027.0 (3,879,174.6) | 5,894,189.3 (3,713,895.4) | 0.4 | >0.9 |
| b_Crotonic.acid | 12 | 16,108,825.9 (23,803,380.1) | 23,947,637.5 (26,242,912.3) | 8,270,014.3 (20,257,315.3) | 0.4 | >0.9 |
| b_Succinic.acid | 12 | 407,804.1 (869,276.7) | 109,080.2 (267,190.7) | 706,528.0 (1,173,394.8) | 0.5 | >0.9 |
| a_L.Lysine | 12 | 24,416,825.1 (28,518,776.0) | 34,566,558.7 (36,434,829.3) | 14,267,091.5 (14,648,122.2) | 0.5 | >0.9 |
| b_Pyridoxal.tent. | 12 | 777,482.7 (1,211,214.3) | 598,171.7 (398,509.7) | 956,793.7 (1,729,598.9) | 0.5 | >0.9 |
| b_X2.C.Methyl.D.erythritol4.phosphate.tent. | 12 | 643,051,857.5 (148,219,463.1) | 606,454,245.0 (172,899,107.7) | 679,649,470.0 (123,382,309.5) | 0.5 | >0.9 |
| b_X2.Methylsuccinic.acid.tent. | 12 | 287,186,644.6 (143,335,650.3) | 264,074,195.0 (155,198,662.0) | 310,299,094.2 (140,821,055.1) | 0.5 | >0.9 |
| b_Pyruvic.acid.tent. | 12 | 150,010,045.5 (9,715,420.7) | 147,364,165.0 (6,350,912.8) | 152,655,926.0 (12,268,697.7) | 0.5 | >0.9 |
| a_Niacinamide | 12 | 1,384,815.1 (2,023,883.3) | 1,614,438.5 (2,373,808.4) | 1,155,191.7 (1,802,752.7) | 0.5 | >0.9 |
| a_X6.Methoxyquinoline.tent. | 12 | 7,956,394.3 (26,119,894.0) | 15,246,370.2 (37,028,395.4) | 666,418.3 (1,516,716.5) | 0.5 | >0.9 |
| a_L..Methionine | 12 | 788,318.3 (1,316,035.4) | 642,601.0 (747,341.7) | 934,035.5 (1,789,080.3) | 0.5 | >0.9 |
| b_X4.Hydroxyquinoline | 12 | 2,294,626.8 (5,198,100.9) | 3,743,521.2 (7,079,171.4) | 845,732.5 (2,071,613.1) | 0.6 | >0.9 |
| a_X2.2.6.6.Tetramethyl.4.piperidinol.tent. | 12 | 3,349,971.7 (5,949,414.1) | 4,474,480.0 (7,959,519.7) | 2,225,463.3 (3,388,416.0) | 0.6 | >0.9 |
| a_N.N.Diethylethanolamine.tent. | 12 | 3,251,751.6 (3,370,326.9) | 2,444,947.7 (2,152,767.5) | 4,058,555.5 (4,335,133.4) | 0.6 | >0.9 |
| b_L.Isoleucine | 12 | 7,328,303.6 (7,854,185.2) | 8,665,141.0 (7,496,666.0) | 5,991,466.2 (8,673,233.1) | 0.7 | >0.9 |
| a_X6.Methylquinoline.tent. | 12 | 385.0 (699.5) | 274.2 (671.6) | 495.8 (772.0) | 0.8 | >0.9 |
| a_Hypoxanthine | 12 | 38,073.7 (83,225.3) | 44,836.3 (109,826.1) | 31,311.0 (55,377.5) | 0.8 | >0.9 |
| b_p.Toluenesulfonic.acid.tent. | 12 | 3,372,499.5 (6,572,551.8) | 3,393,943.8 (5,259,090.6) | 3,351,055.2 (8,208,375.3) | 0.8 | >0.9 |
| b_Urocanic.acid.tent. | 12 | 1,399.0 (2,534.9) | 1,818.7 (2,821.3) | 979.3 (2,398.9) | 0.8 | >0.9 |
| a_Pyridoxal.tent. | 12 | 1,928,431.0 (3,031,562.9) | 2,203,965.7 (3,922,077.7) | 1,652,896.3 (2,157,295.3) | 0.8 | >0.9 |
| b_X3.Methyl.2.oxovaleric.acid | 12 | 1,101,172.5 (3,039,252.9) | 282,158.3 (278,456.3) | 1,920,186.7 (4,316,724.3) | 0.8 | >0.9 |
| b_L.Methionine | 12 | 747,354.3 (822,900.9) | 676,740.7 (845,132.3) | 817,968.0 (873,813.0) | 0.8 | >0.9 |
| b_L.Tryptophan | 12 | 198,603.2 (296,935.2) | 272,248.2 (407,584.4) | 124,958.2 (121,792.6) | 0.8 | >0.9 |
| a_Indole.3.acrylic.acid | 12 | 2,553,283.9 (3,140,722.0) | 2,083,026.7 (2,110,564.8) | 3,023,541.2 (4,088,504.8) | 0.8 | >0.9 |
| b_X4.Dodecylbenzenesulfonic.acid.tent. | 12 | 10,280,591.5 (7,701,423.0) | 8,121,727.2 (4,928,645.2) | 12,439,455.8 (9,747,256.3) | 0.8 | >0.9 |
| a_N.Acetylputrescine | 12 | 386,960.3 (468,563.5) | 391,056.2 (550,659.6) | 382,864.3 (423,966.5) | 0.9 | >0.9 |
| a_D..Proline | 12 | 661,884.2 (1,149,335.2) | 957,007.0 (1,564,926.8) | 366,761.3 (498,103.3) | 0.9 | >0.9 |
| a_X4.Aminonicotinic.acid.or.isomer. | 12 | 126,303.5 (225,453.7) | 94,034.3 (196,574.4) | 158,572.7 (265,864.7) | >0.9 | >0.9 |
| a_Choline | 12 | 174,331,021.6 (21,492,889.5) | 171,722,892.2 (20,170,821.4) | 176,939,151.0 (24,353,435.2) | >0.9 | >0.9 |
| b_Acetoacetic.acid | 12 | 319,609,159.2 (14,397,923.5) | 321,712,028.7 (13,338,036.2) | 317,506,289.7 (16,356,781.0) | >0.9 | >0.9 |
| a_Isoleucine | 12 | 30,943,808.3 (15,505,575.1) | 29,880,732.5 (15,885,905.7) | 32,006,884.2 (16,548,594.4) | >0.9 | >0.9 |
| b_L.Phenylalanine | 12 | 9,511,810.9 (5,608,112.4) | 10,045,803.3 (6,593,135.4) | 8,977,818.5 (5,003,823.0) | >0.9 | >0.9 |
| a_Creatine | 12 | 137,229.8 (326,719.4) | 112,430.3 (275,396.9) | 162,029.2 (396,888.8) | >0.9 | >0.9 |
| b_D..Fructose.or.isomer. | 12 | 1,376,324,172.0 (124,013,739.0) | 1,382,734,756.2 (112,787,783.2) | 1,369,913,587.8 (144,965,454.4) | >0.9 | >0.9 |
| b_Folic.acid | 12 | 3,351,599.5 (3,207,090.2) | 4,052,769.5 (4,167,729.0) | 2,650,429.5 (2,019,416.2) | >0.9 | >0.9 |
| b_Propylparaben.or.isomer. | 12 | 255,003.3 (618,051.8) | 190,425.0 (466,444.1) | 319,581.7 (782,812.0) | >0.9 | >0.9 |
*
Mean (SD).
†
Wilcoxon rank sum test; Wilcoxon rank sum exact test.
‡
False discovery rate correction for multiple testing.