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. 2024 Nov 22;62(4):399–407. doi: 10.3347/PHD.24062

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© 2024 The Korean Society for Parasitology and Tropical Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2 — Real-time PCR analysis of differentially expressed genes (DEGs) in A. castellanii. mRNA expression levels of 5 DEGs in A. castellanii during encystation and excystation were assessed using real-time PCR. (A) ACA1_031140. (B) ACA1_032330. (C) ACA1_374400. (D) ACA1_275740. (E) ACA1_112650. The transcription levels of these 5 genes were compared among Acanthamoeba trophozoites and encysting and excysting forms. Data are shown as the mean±SD, representing 3 independent experiments. Asterisks indicate statistical significance: ****P<0.0001 compared to Acanthamoeba trophozoites.