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Real-time PCR analysis following siRNA transfection. Acanthamoeba castellanii were transfected with siRNAs targeting the 5 DEGs. After 48 h of encystation, excystation was immediately induced for 8 h, and gene knockdown was examined. (A) ACA1_031140. (B) ACA1_032330. (C) ACA1_374400. (D) ACA1_275740. (E) ACA1_112650. Data are presented as the mean±SD, representative of 3 independent experiments. ****P<0.0001 between the control group (□) and the siRNA-transfected group (■).
