Figure 2.
Cd and Mn uptake efficiencies of M235X mutants of OsNramp5. (A) Cd uptake efficiency was estimated for 19 M235X mutants in the yeast assay system, together with the previously reported mutants of Q337K and A512T. This test was conducted using Δycf1 in the presence of 0 µM to 81.8 µM CdSO4. The relative Cd uptake was estimated as shown in Supplementary Figure 2 . The mean and standard deviation of relative Cd uptake efficiency are shown (N=3, WT=100%). The mutants exhibited <20% Cd and >60% Mn uptake efficiencies are highlighted in red. (B) Mn uptake efficiencies are shown as in (A). This test was conducted using Δpmr1 in the presence of 0 mM to 1.5 mM MnSO4.