Figure 3.
Cd and Mn uptake efficiencies of OsNramp5 double mutants. (A) Cd uptake efficiency was estimated for eight double mutants in the yeast assay system, together with four single mutants at residue 232. M235A and M235C were also used as references. This test was conducted using Δycf1 in the presence of 0 µM to 81.8 µM CdSO4. The relative uptake value was calculated using the apparent metal uptake of the empty plasmid and WT as 0% and 100%, respectively. The mean and standard deviation of relative Cd uptake efficiency are shown (N=3, WT=100%). The mutants exhibited <20% Cd and >60% Mn uptake efficiencies are highlighted in red. B) Mn uptake efficiencies are shown as in (A). This test was conducted using Δpmr1 in the presence of 0 mM to 1.5 mM MnSO4.