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Cd and Mn uptake in various Cd concentrations. (A) Apparent Cd content measured using ICP-OES for the WT yeast strain (BY4741) containing OsNramp5 (WT) and the mutants (M235A, A232C+M235A, and A232S+M235A) in the presence of different Cd concentrations (0, 1, 2.5, and 7.5 μM) and fixed 1.8 mM MnSO4. The mean and standard deviation were shown (N = 3). (B) Apparent Mn content measured in the same condition as (A).
