Figure 5.

Structure and chemical properties that may determine the Cd and Mn uptake efficiency of OsNramp5. (A) Appropriate tunnel length, tunnel minimum radius, and hydropathy around the metal-binding site hypothesized to be required for efficient Mn uptake (>60% of WT). The number of mutants that passed in the node is shown. (B) Structure of the predicted transport tunnel and metal-binding site of WT, M235A, M235P, M235V, and M235S. The predicted tunnels are visualized by blue mesh, together with the key residues. The predicted cavity around the metal-binding site is shown by a small sphere, which is colored according to the Eisenberg & Weiss hydropathy scale. The properties of the length (L) and minimum radius (M) of the transport tunnel, and hydropathy around the metal-binding site (H), are shown.