Table 1.
Relative Cd and Mn uptake efficiency and Mn/Cd ratio of OsNramp5 M235X mutants.
| Mn/Cd ratio |
Relative Cd uptake (%) | Relative Mn uptake(%) | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Mean | ± | SD | p-value | Mean | ± | SD | p-value | ||
| Mock | – | 0.0 | ± | 0.0 | ** | 0.0 | ± | 0.0 | ** |
| WT | 1.0 | 100.0 | ± | 0.0 | 100.0 | ± | 0.0 | ||
| Q337K | 1.0 | 36.0 | ± | 11.9 | ** | 37.2 | ± | 16.8 | ** |
| A512T | 0.9 | 53.9 | ± | 5.5 | ** | 46.3 | ± | 17.8 | ** |
| M235A | 11.3 | 7.8 | ± | 0.5 | ** | 88.5 | ± | 12.4 | |
| M235C | 7.5 | 8.6 | ± | 0.8 | ** | 65.0 | ± | 30.4 | |
| M235N | 2.5 | 6.7 | ± | 1.5 | ** | 16.7 | ± | 41.2 | ** |
| M235D | 1.8 | 13.4 | ± | 4.5 | ** | 24.6 | ± | 32.4 | ** |
| M235L | 1.8 | 8.9 | ± | 0.4 | ** | 16.2 | ± | 18.8 | ** |
| M235H | 1.7 | 11.3 | ± | 0.8 | ** | 19.7 | ± | 30.9 | ** |
| M235K | 1.0 | 8.1 | ± | 1.1 | ** | 8.5 | ± | 37.3 | ** |
| M235E | 1.0 | 11.0 | ± | 3.5 | ** | 11.2 | ± | 45.9 | ** |
| M235G | 1.0 | 8.3 | ± | 0.2 | ** | 8.0 | ± | 48.4 | ** |
| M235T | 0.6 | 14.6 | ± | 0.3 | ** | 9.3 | ± | 14.6 | ** |
| M235S | 0.5 | 9.9 | ± | 1.5 | ** | 4.9 | ± | 19.4 | ** |
| M235W | -0.1 | 17.8 | ± | 1.3 | ** | -1.6 | ± | 7.2 | ** |
| M235I | -0.7 | 13.4 | ± | 0.5 | ** | -9.1 | ± | 23.1 | ** |
| M235Q | -0.9 | 12.4 | ± | 1.0 | ** | -11.1 | ± | 48.8 | ** |
| M235V | -0.9 | 11.2 | ± | 2.3 | ** | -10.5 | ± | 16.9 | ** |
| M235P | -1.3 | 12.8 | ± | 1.4 | ** | -17.0 | ± | 20.5 | ** |
| M235F | -1.4 | 10.0 | ± | 2.7 | ** | -13.7 | ± | 26.2 | ** |
| M235Y | -1.4 | 11.5 | ± | 2.4 | ** | -16.3 | ± | 2.2 | ** |
| M235R | -3.7 | 8.3 | ± | 0.9 | ** | -30.4 | ± | 32.7 | ** |
The results shown in Figure 2 were analyzed based on the relative Cd/Mn uptake (%) and Mn/Cd ratio. The mean and standard deviation (SD) are shown (N=3) with the statistical evaluation compared to the WT using Dunnett’s test (** p <0.01).