Table 2.
Relative Cd and Mn uptake efficiency and Mn/Cd ratio of OsNramp5 single and double mutants.
| Mn/Cd ratio |
Relative Cd uptake (%) | Relative Mn uptake (%) | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Mean | ± | SD | p-value | Mean | ± | SD | p-value | ||
| Mock | – | 0.0 | ± | 0.0 | ** | 0.0 | ± | 0.0 | ** |
| WT | 1.0 | 100.0 | ± | 0.0 | ** | 100.0 | ± | 0.0 | |
| M235A | 11.3 | 7.8 | ± | 0.5 | 88.5 | ± | 12.4 | ||
| M235C | 7.5 | 8.6 | ± | 0.8 | 65.0 | ± | 30.4 | ||
| A232S+M235A | 17.0 | 5.7 | ± | 1.7 | 96.4 | ± | 4.6 | ||
| A232S+M235C | 13.6 | 4.7 | ± | 0.4 | 64.1 | ± | 12.4 | ||
| A232C+M235A | 12.8 | 5.5 | ± | 1.5 | 69.8 | ± | 12.5 | ||
| A232M | 6.2 | 6.8 | ± | 0.3 | 42.3 | ± | 6.0 | ** | |
| A232C+M235C | 6.0 | 7.3 | ± | 0.5 | 43.7 | ± | 15.8 | ** | |
| A232V | 5.0 | 8.7 | ± | 1.7 | 43.1 | ± | 34.7 | ** | |
| A232S | 2.2 | 20.0 | ± | 1.9 | ** | 44.0 | ± | 15.8 | ** |
| A232C | 1.5 | 27.8 | ± | 3.5 | ** | 42.5 | ± | 13.9 | ** |
| A232V+M235C | 1.5 | 12.8 | ± | 2.2 | ** | 19.1 | ± | 5.1 | ** |
| A232V+M235A | 1.3 | 11.2 | ± | 7.8 | 14.5 | ± | 11.2 | ** | |
| A232M+M235A | 0.8 | 16.1 | ± | 0.9 | ** | 12.1 | ± | 12.9 | ** |
| A232M+M235C | -1.1 | 9.6 | ± | 0.8 | -10.7 | ± | 1.9 | ** | |
The results shown in Figure 3 were analyzed based on the estimated relative Cd/Mn uptake (%) and Mn/Cd ratio. The mean and standard deviation (SD) are shown (N=3) with statistical evaluation compared to M235A using Dunnett’s test (** p <0.01).