Table 3.
Direct measurement of Cd and Mn content for selected OsNramp5 mutants.
| Construct | Mn/Cd ratio |
Cd content | Mn content | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Relative uptake value (%) |
Mean (ng/mg) |
± | SD | p-value | Relative uptake value (%) |
Mean (ng/mg) |
± | SD | p-value | |||
| Mock | – | 0.0 | 15.5 | ± | 1.9 | ** | 0.0 | 7.5 | ± | 0.8 | ** | |
| WT | 1.0 | 100.0 | 129.9 | ± | 10.0 | 100.0 | 17.0 | ± | 2.4 | |||
| A512T | 0.5 | 90.4 | 118.9 | ± | 19.6 | 48.7 | 12.2 | ± | 1.5 | ** | ||
| M235A | 3.5 | 17.2 | 35.3 | ± | 3.5 | ** | 60.2 | 13.2 | ± | 1.2 | * | |
| M235C | 5.0 | 10.9 | 28.0 | ± | 3.3 | ** | 54.8 | 12.7 | ± | 1.0 | * | |
| M232C+M235A | 7.4 | 13.9 | 31.4 | ± | 4.9 | ** | 103.3 | 17.3 | ± | 0.7 | ||
| M232S+M235A | 3.7 | 22.3 | 41.0 | ± | 5.0 | ** | 82.8 | 15.4 | ± | 0.8 | ||
| M232S+M235C | 4.6 | 13.5 | 31.0 | ± | 7.1 | ** | 62.5 | 13.5 | ± | 2.1 | * | |
The Cd/Mn content of the yeast was measured using ICP-OES. The results were analyzed based on the relative Cd/Mn uptake (%) and Mn/Cd ratio. Relative uptake values were calculated using the apparent metal content of the empty plasmid and WT as 0% and 100%, respectively. The mean and standard deviation (SD) are shown (N=3) with the statistical evaluation compared to the WT using Dunnett’s test (*p<0.05, ** p <0.01).