Table 4.

Apparent Cd and Mn uptake in various Cd concentrations for selected OsNramp5 mutants.

Construct	CdSO4	Apparent Cd content (ng/mg)					Apparent Mn content (ng/mg)
		Relative uptake value (%)	Mean	±	SD	p-value	Relative uptake value (%)	Mean	±	SD	p-value
Mock	0 µM	–	N.D.				0.0	0.0	±	0.0	**
	2.5 µM	0.0	0.0	±	0.0		0.0	0.0	±	0.0	**
	7.5 µM	0.0	0.0	±	0.0	**	0.0	0.0	±	0.0	**
WT	0 µM	–	N.D.				100.0	298.0	±	59.3
	2.5 µM	100.0	2.0	±	1.5		100.0	433.1	±	42.5
	7.5 µM	100.0	16.7	±	2.8		100.0	501.9	±	67.9
M235A	0 µM	–	N.D.				104.4	311.1	±	37.3
	2.5 µM	0 >	-1.9	±	0.9	*	82.0	355.3	±	5.8	**
	7.5 µM	0 >	-2.0	±	2.5	**	76.1	381.7	±	13.3
A232C+M235A	0 µM	–	N.D.				91.9	273.9	±	41.6
	2.5 µM	0 >	-1.4	±	0.7		84.0	363.6	±	21.9	*
	7.5 µM	0 >	-6.5	±	5.0	**	64.7	324.8	±	98.2	**
A232S+M235A	0 µM	–	N.D.				107.9	321.5	±	56.9
	2.5 µM	0 >	-3.0	±	2.8	**	79.8	345.6	±	26.6	**
	7.5 µM	0 >	-3.6	±	4.2	**	68.5	343.7	±	14.4	*

The results in Figure 4 were analyzed based on the relative Cd and Mn uptake (%). Relative uptake values were calculated using the apparent metal content of the empty plasmid and WT as 0% and 100%, respectively. The mean and standard deviation (SD) are shown (N=3) with the statistical evaluation compared to the WT using Dunnett’s test (*p<0.05, **p<0.01).

N.D., Not Detected.