Fig. 2 – Single-cell m⁶A profiling in the mouse cortex.

a, UMAP visualization of cell types identified in the adult mouse cortex. b, Metagene analysis of m⁶A sites identified by scDART-seq across all cell types in the mouse cortex. c, Metagene analysis of m⁶A sites identified within each cell type classification. Cell types represented by fewer than 100 cells are omitted. d, Number of m⁶A sites identified in each cell type normalized by the total number of reads in the cell. Red dot indicates the mean. Significance was determined using a two-sided Wilcoxon rank-sum test comparing microglia to all other cells. **** = p < 0.0001. p = 2.73e−14. e, UPLC-MS data showing decreased m⁶A/A ratio in mRNA purified from microglia compared to astrocytes. n = 2 biological replicates. Glut = Glutamatergic Neuron; GABA = GABAergic Neuron; Oligo = Oligodendrocyte; Micro = Microglia; CR = Cajal-Retzius Cell; Macro = Peripheral Macrophage; SMC = Smooth Muscle Cell.