Extended Data Fig. 1 – Tamoxifen-inducible APOBEC1-YTH expression in DART mice.

a, Western blots from liver, lung, kidney, and heart showing inducible transgene expression in APOBEC1-YTH mice (DART mice; top row) and APOBEC1-YTH^mut mice (bottom row). Blots are representative of n = 2 biological replicates. GAPDH was run concurrently on a separate identical blot. b, Immunofluorescence of APOBEC1-YTH expression in a coronal DART mouse brain section. Expression is induced across all layers of the cortex. Images are representative of data from n = 2 vehicle-treated and n = 3 tamoxifen-treated mice, each biological replicates). c, Western blot comparing APOBEC1-YTH expression after different induction protocols. Blots are representative of n = 2 biological replicates. GAPDH was run concurrently on a separate identical blot. d, Quantification of %C2U adjacent to Zhx1 A2295 after each induction protocol (n = 2 biological replicates for 4 days tamoxifen +1 and 5 days tamoxifen + 3; n =3 biological replicates for 5 days tamoxifen + 7). e, Western blot detecting METTL3 from the cortex of mice treated with corn oil or tamoxifen. Image is representative of n = 3 biological replicates. GAPDH was run concurrently on a separate identical blot. f, Quantification of western blots from (f). n = 3 biological replicates. Corn oil mean = 1. Tamoxifen mean 1.18. p = 0.44. Error bars represent standard deviation.