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. Author manuscript; available in PMC: 2024 Dec 4.
Published in final edited form as: Nat Protoc. 2024 Aug 8;19(12):3750–3776. doi: 10.1038/s41596-024-01033-8

Fig. 2 |. Sample preparation and MS throughput.

Fig. 2 |

a, Depending on the multiplexed scheme selected, the user can prepare between 1,280 and 3,712 cells for analysis in a single sample preparation. The number of cells that is possible in practice depends on the spacing on the slide. At lower levels of multiplexing, there are more multiplexed sets to pick up; at some point, the pickup takes too long to be practical. b, The number of single cells analyzed per day by LC-MS/MS for 3-plexDIA (100 cells/d) and by pSCoPE (1,018 cells/d). LC-MS/MS throughput could be further increased by methods that obviate sample loading and column washing overheads such as the use of trapping columns or EvoSep.