Fig. 4 |. Evaluating head camera images of slides.

a, A head camera image taken of a single field. Each slide contains four replicate fields. Each field contains a quality spot (1. DMSO, 2. cell, 3. digestion mix) that indicates if a reagent-dispensing issue occurred mid-run. b, The user may also be able to identify failed dispensing if the reagent misses the droplets. c, A successful dispensing is often indicated by a consistent increase in the size of the reaction droplet from pre-dispensing images to post-dispensing images and should not show smaller droplets to the side of primary spots. d, An after-image of failed pickup shows significant residue left behind on the slide. e, An after-image of a successful pickup shows little to no residue left on the slide.