. Author manuscript; available in PMC: 2024 Dec 4.

Published in final edited form as: Nat Protoc. 2024 Aug 8;19(12):3750–3776. doi: 10.1038/s41596-024-01033-8

Table 2 |.

Comparison of sample-preparation methods for multiplexed single-cell proteomics

	Method and category
	mPOP (multiwell plate)	ProteoCHIP (multiwell chip)	N2 (multiwell chip)	nPOP (coated glass slide)
Volume	1 μl	100 nl	10–30 nl	10–30 nl
Requires CellenONE	No	Yes	Yes	Yes
Multiplexing specific consumable	No	Yes	Yes	No
Multiplexed applications (demonstrated in publication)	11, 16^6,29,47	11, 16¹²	11, 32^48,49	3, 18, 32^7,17
Cells prepared in parallel (demonstrated in publication)	384⁶	170¹²	164⁴⁹	2,016⁷
Cells prepared per experiment (theoretical, based on published designs)	384⁶	192¹²	3,456⁴⁹	3,712

The table includes only methods that have been used for preparing single-cell peptide samples labeled with mass tags analyzed by multiplexed LC-MS/MS.