Table 4 |.
Label loadings into the probe plate
| Well position | 2-plex (mTRAQ, 20 μl) | 3-plex (mTRAQ, 20 μl) | 14-plex (TMT, 30 μl) | 29-plex (TMT, 30 μl) |
|---|---|---|---|---|
| G1 | d0 (slide 1) | d0 (slide 1) | TMTpro-128C | TMTpro-127D |
| G2 | d4 (slide 1) | d4 (slide 1) | TMTpro-129N | TMTpro-128N |
| G3 | d0 (slide 2) | d8 (slide 1) | TMTpro-129C | TMTpro-128ND |
| G4 | d4 (slide 2) | d0 (slide 2) | TMTpro-130N | TMTpro-128C |
| G5 | d0 (slide 3) | d4 (slide 2) | TMTpro-130C | TMTpro-128CD |
| G6 | d4 (slide 3) | d8 (slide 2) | TMTpro-131N | TMTpro-129N |
| G7 | d0 (slide 4) | d0 (slide 3) | TMTpro-131C | TMTpro-129ND |
| G8 | d4 (slide 4) | d4 (slide 3) | TMTpro-132N | TMTpro-129C |
| G9 | - | d8 (slide 3) | TMTpro-132C | TMTpro-129CD |
| G10 | - | d0 (slide 4) | TMTpro-133N | TMTpro-130N |
| G11 | - | d4 (slide 4) | TMTpro-133C | TMTpro-130ND |
| G12 | - | d8 (slide 4) | TMTpro-134N | TMTpro-130C |
| G13 | - | - | TMTpro-134C | TMTpro-130CD |
| G14 | - | - | TMTpro-135N | TMTpro-131N |
| G15 | - | - | - | TMTpro-131ND |
| G16 | - | - | - | TMTpro-131C |
| G17 | - | - | - | TMTpro-131CD |
| G18 | - | - | - | TMTpro-132N |
| G19 | - | - | - | TMTpro-132ND |
| G20 | - | - | - | TMTpro-132C |
| G21 | - | - | - | TMTpro-132CD |
| G22 | - | - | - | TMTpro-133N |
| G23 | - | - | - | TMTpro-133ND |
| G24 | - | - | - | TMTpro-133C |
| H1 | - | - | - | TMTpro-133CD |
| H2 | - | - | - | TMTpro-134N |
| H3 | - | - | - | TMTpro-134ND |
| H4 | - | - | - | TMTpro-134C |
| H5 | - | - | - | TMTpro-135N |
The number of slides and multiplexing scheme chosen will dictate the number of single cells prepared in one experiment. If fewer than four slides are to be processed in a plexDIA experiment, load only wells correlating to the number of slides processed.