Table 4.
Summary of the number of EV-miRNA associated with HMOs measures and concentrations via multivariable linear regression analysis.
| HMO Summary Measures (nmol/mL) | P < 0.05 Count |
PBH < 0.10 Count |
|---|---|---|
| Diversity+ | 26 | – |
| Sum of HMOs | 55 | – |
| HMO-bound sialic acid | 9 | – |
| HMO-bound fucose | 33 | – |
| HMO Concentrations (nmol/mL) | P < 0.05 Count |
PBH < 0.10 Count |
| 2’FL | 52 | 7 |
| 3’FL | 63 | 17 |
| 3’SL | 34 | 1 |
| 6’SL | 52 | 1 |
| DFLac | 34 | 0 |
| DFLNH | 7 | 0 |
| DFLNT | 39 | 0 |
| DSLNH | 20 | 0 |
| DSLNT | 34 | 0 |
| FDSLNH | 26 | 0 |
| FLNH | 52 | 4 |
| LnNT | 21 | 0 |
| LNT | 29 | 0 |
| LNFP I | 29 | 1 |
| LNFP II | 22 | 0 |
| LNFP III | 10 | 0 |
| LNH | 58 | 5 |
| LSTb | 19 | 0 |
| LSTc | 13 | 0 |
Multivariable linear regression analysis was used to examine the associations between individual EV-miRNAs with HMO summary measures and HMO concentrations. Models adjusted for adjusted for technical covariates (i.e., proportion of rRNA, volume of skim milk) as well as days postpartum, human milk collection time, and breast feedings per day. “P < 0.05 Count” indicates the number of EV-miRNAs that were associated with each HMO measure based on P < 0.05. “PBH Count” indicates the number of EV-miRNAs that were associated with each HMO measures based on PBH < 0.10.
+Diversity was estimated using Simpson’s Diversity measure.