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Nitrate supplementation accelerated angiogenesis and increases blood perfusion. (A, C–E) Immunohistochemical staining of VEGF, bFGF and CD31 on day 14 after wound healing and their quantitative results. Scale bar = 50 μm. (B, F) Immunofluorescence staining of α-SMA at day 14 post-wounding and its quantitative results. Scale bars = 100 μm. (G) Blood perfusion change rates at day 7 and 14 post-wounding. n = 4, * P < 0.05, ** P < 0.01, *** P < 0.001.
