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Longitudinal plots showing ctDNA changes over time in 19 patients with at least one positive ctDNA collection. The red dots indicate positive for ctDNA collections while the black dots indicate negative for ctDNA collections. The time of definitive surgery is indicated by a blue line while the time of local and systemic progression corresponds to the orange and red dashed lines, respectively. ctDNA, circulating tumor DNA; eVAF, estimated variant allele fraction.
