Fig. 7.

DC-Y13-27 diminished the effect of H₂O₂ on the expression of FOXO3/TIMP1/MMPs in vitro. (A) Changes in intracellular ROS levels in NP cells following treatment with H₂O₂ (0 and 150 µM) and DC-Y13-27 (0, 20, and 40 µM) for 6 h. ROS levels were quantified using DCFH-DA staining. (B) Protein expression levels of YTHDF2, FOXO3, Med1, CBP, TIMP1, MMP1, MMP3, MMP7, and MMP9 in NP cells after treatment with H₂O₂ and DC-Y13-27. (C) RIP assay to detect FOXO3 mRNA association with YTHDF2 in NP cells treated with H₂O₂ and DC-Y13-27 (DC), using IgG and anti-YTHDF2 antibodies. (D) Assessment of the binding dynamics of the Med1-CBP-FOXO3 complex on the TIMP1 promoter in NP cells after treatment with H₂O₂ and DC-Y13-27 (DC). ns represents no significant difference. *P < 0.05, **P < 0.01, ***P < 0.001