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. 2024 Sep 16;27(10):110980. doi: 10.1016/j.isci.2024.110980

Figure 2.

Figure 2

Effect of LRRK2 inhibition on mitophagy pathway in brain

Cytosolic protein fractions of brain tissues were used to evaluate mitophagic protein expression such as OPT, LAMP2, and p62 and pro-inflammatory cytokine TNF-α after MPTP intoxication and PF-475 treatments. Representative western blot of mitophagic protein are shown (A; p = 0.09; F(4,45) = 2.16, one-way ANOVA method, followed by Bonferroni post hoc test for multiple comparisons), (B; p = 0.04; F (4,45) = 2.77, one-way ANOVA method, followed by Bonferroni post hoc test for multiple comparisons) and (C; p = 0.11; F(4,45) = 2.02, one-way ANOVA method, followed by Bonferroni post hoc test for multiple comparisons), see densitometric analysis (A1, B1 and C1, respectively). Western blot of pro-inflammatory cytokine TNF-α are shown (D; p = 0.16; F(4,45) = 1.86, one-way ANOVA method, followed by Bonferroni post hoc test for multiple comparisons), see densitometric analysis (D1). Data are representative of at least three independent experiments. Values are means ± SD. Distribution of values come from individual animals. One-way ANOVA test. ∗∗∗p < 0.001 vs. control; ##p < 0.01 vs. MPTP; ###p < 0.001 vs. MPTP.