Figure 4.

Effect of PF-475 on pro-inflammatory response induced by MPTP intoxication in colon tissues

Representative western blot of pro-inflammatory enzyme iNOS and COX-2 in cytosolic fraction of colon tissues after MPTP and PF-475 treatments (A; p = 0.005, F(4,45) = 4.25, one-way ANOVA method, followed by Bonferroni post hoc test for multiple comparisons) (B; p = 0.07, F(4,45) = 2.31, one-way ANOVA method, followed by Bonferroni post hoc test for multiple comparisons), see densitometric analysis (A1, B1, respectively). ELISA method was performed for detection of IL-1β in cytosolic fraction of colon tissues (C). Data are representative of at least three independent experiments. Values are means ± SD. Distribution of values come from individual animals. One-way ANOVA test. ∗p < 0.05 vs. control ∗∗∗p < 0.001 vs. control; ##p < 0.01 vs. MPTP; ###p < 0.001 vs. MPTP.