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. 2024 Sep 16;27(10):110980. doi: 10.1016/j.isci.2024.110980

Figure 5.

Figure 5

PF-475 treatment preserved α-syn accumulation in colon tissue

Immunohistochemical localization of α-syn after MPTP in colon tissue. Control group (A, A1; see score F), MPTP group (B, B1; see score F), PF-475 2.5 mg/kg treatments after MPTP (C, C1; see score F), PF-475 5 mg/kg treatments after MPTP (D, D1; see score F) and PF-475 10 mg/kg treatments after MPTP (E, E1; see score F; p = 0.4; F(4,45) = 2.84, one-way ANOVA method, followed by Bonferroni post hoc test for multiple comparisons). Representative western blot of α-syn tetramer and monomer protein expression level in colon tissues after MPTP intoxication and PF-475 treatments (G and H; see densitometric analysis G1, H1, respectively; p = 0.001; F (4,45) = 1.25, one-way ANOVA method, followed by Bonferroni post hoc test for multiple comparisons; p = 0.001; F (4,45) = 2.81, respectively for G1 and H1). Representative Western blot of total α-syn protein expression level in colon tissues after MPTP intoxication and PF-475 treatments (densitometric analysis I; p = 0.001; F (4,45) = 5.51, one-way ANOVA method, followed by Bonferroni post hoc test for multiple comparisons). Data are representative of at least three independent experiments. Values are means ± SD. Distribution of values come from individual animals. One-way ANOVA test. ∗∗∗p < 0.001 vs. control; ##p < 0.01 vs. MPTP; ###p < 0.001 vs. MPTP.