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. 2024 Sep 16;27(10):110980. doi: 10.1016/j.isci.2024.110980

Figure 6.

Figure 6

Effect of PF-475 on ENS in MPTP-injected mouse colon

Representative western blot of ENS proteins such as PGP9.5 and NSE in cytosolic fraction of colon tissues after MPTP and PF-475 treatments (A; p = 0.98; F (4,45) = 0.110, one-way ANOVA method, followed by Bonferroni post hoc test for multiple comparisons) (B; p = 0.36; F (4,45) = 1.12, one-way ANOVA method, followed by Bonferroni post hoc test for multiple comparisons), see densitometric analysis (A1, B1, respectively). Immunohistochemical localization of PGP9.5 after MPTP intoxication in colon tissue. Control group (C, C1; see score H), MPTP group (D, D1; see score H), PF-475 2.5 mg/kg treatments after MPTP (E, E1; see score H), PF-475 5 mg/kg treatments after MPTP (F, F1; see score H; p = 0.45; F (4,45) = 0.941, one-way ANOVA method, followed by Bonferroni post hoc test for multiple comparisons) and PF-475 10 mg/kg treatments after MPTP (G, G1; see score F). Data are representative of at least three independent experiments. Values are means ± SD. Distribution of values come from individual animals. One-way ANOVA test. ∗∗ <0.01 vs. control; ∗∗∗p < 0.001 vs. control; #p < 0.05 vs. MPTP; ##p < 0.01 vs. MPTP; ###p < 0.001 vs. MPTP.