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. 2024 Sep 16;27(10):110980. doi: 10.1016/j.isci.2024.110980

Figure 7.

Figure 7

Effect of PF-475 on mitophagy pathway in MPTP-injected mouse colon

Cytosolic fractions of colon tissues were used to evaluate mitophagic protein expression such as OPT, LAMP2 and p62 and pro-inflammatory cytokine TNF-α after MPTP intoxication and PF-475 treatments. Representative western blot of mitophagic protein are shown (A–C), see densitometric analysis of OPT (A1; p = 0.28; F (4,45) = 1.33, one-way ANOVA method, followed by Bonferroni post hoc test for multiple comparisons) LAMP2 (B1; p = 0.31; F (4,45) = 1,23, one-way ANOVA method, followed by Bonferroni post hoc test for multiple comparisons) and p62 (C1; p = 0.79; F (4,45) = 0.420, one-way ANOVA method, followed by Bonferroni post hoc test for multiple comparisons). Expression levels of pro-inflammatory cytokine TNF-α are shown (D, see densitometric analysis D1; p = 0.44; F (4,45) = 0.957, one-way ANOVA method, followed by Bonferroni post hoc test for multiple comparisons). Data are representative of at least three independent experiments. Values are means ± SD. Distribution of values come from individual animals. One-way ANOVA test. ∗∗∗p < 0.001 vs. control; #p < 0.05 vs. MPTP; ###p < 0.001 vs. MPTP.