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. 2024 Sep 16;27(10):110980. doi: 10.1016/j.isci.2024.110980

Figure 8.

Figure 8

Immunofluorescence analysis of occludin and ZO-1

Colon sections were stained with antibodies against occludin (green) or ZO-1 (green) and DAPI to highlight cell nuclei (blue). Occludin + cells in control group (A, A1); see mean of intensity fluorescence (F). No Occludin + cells were showed in mice subjected to MPTP (B, B1); see mean of intensity fluorescence (F). Mice treated with PF-475 at doses of 2.5 mg/kg (C, C1) see mean of intensity fluorescence (F), and 5 mg/kg (D, D1); see mean of intensity fluorescence (F) and 10 mg/kg (E, E1); see mean of intensity fluorescence (F; p = 0.89; F (4,45) = 0.277, one-way ANOVA method, followed by Bonferroni post hoc test for multiple comparisons). ZO-1 + cells in control group (G, G1); see mean of intensity fluorescence (L). No ZO-1+ cells were showed in mice subjected to MPTP (H, H1); see mean of intensity fluorescence (L). Mice treated with PF-475 at doses of 2.5 mg/kg (I, I1) see mean of intensity fluorescence (L), and 5 mg/kg (J, J1); see mean of intensity fluorescence (L) and 10 mg/kg (K, K1); see mean of intensity fluorescence (L; p = 0.85; F (4,45) = 0.341, one-way ANOVA method, followed by Bonferroni post hoc test for multiple comparisons). Constipation-related indicator was assessed through determination of fecal weight (M). Data are representative of at least three independent experiments. Values are means ± SD. Distribution of values come from individual animals. One-way ANOVA test. ∗∗∗p < 0.001 vs. control; ##p < 0.01 vs. MPTP; ###p < 0.001 vs. MPTP.