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. 2024 Dec 4;15:10545. doi: 10.1038/s41467-024-54836-4

Fig. 6. Cancer-associated GAIN domain mutant shifts the smFRET peaks to higher FRET.

Fig. 6

a Model of ADGRL3 showing positions of residues S810/E811 in purple, with potential interaction residues from extracellular loops of 7TM shown as sticks (blue). b Compilation of smFRET population histograms of ADGRL3 for the sensor Y795:A871 UAA and for mutant S810L/E811Q with or without 1 µM LK1 and ADGRL3 WT. c smFRET population histogram of ADGRL3 mutant S810L/E811Q fitted to three Gaussian distributions (gray) centered at 0.44, 0.55, and 0.65. The cumulative fit is purple. d Number of states (%) occurring in individual traces from WT ADGRL3 and S810L/E811Q mutant. e Occupancy of the three FRET states in WT and S810L/E811Q mutant ADGRL3. f Occurrence of the three individual FRET states (0.44, 0.55, and 0.65) among single-state traces. g smFRET population histogram of ADGRL3 mutant S810L/E811Q in a presence of 1 µM LK1 antibody. Histogram was fitted to three Gaussian distributions (gray) with centers at 0.44, 0.55, and 0.65. The blue line is the cumulative fit. h Change in the occupancy of the three FRET states after addition of 1 µM LK1 to ADGRL3 mutant S810L/E811Q i Change in the percentage of different FRET states among all traces that stayed in a single state throughout the recording in the presence of 1 µM LK1 compared to apo receptor. b–i Data represent mean ± s.e.m. (n = 3), of three independent biological replicates. Source data are provided as a Source Data file.