Skip to main content
. 2024 Dec 4;15:10543. doi: 10.1038/s41467-024-54850-6

Fig. 4. OLFM4 high expression positively correlated with EBV infection in gastric cancer.

Fig. 4

a Representative image of EBV fluorescence in situ hybridization (FISH) from 168 clinical GC samples. EBER-1 RNA is used as the detection marker for EBV infection. Scale bars, 100 μm. b Pie chart showing t EBV infection rate. −: Negative (≤1 EBER-1 signal point); +: positive (2–100 EBER-1 signal points); ++: strong positive (>100 EBER-1 signal points). c Pie chart showing the tumor site preference of EBV positive gastric cancers. d Representative images for OLFM4 staining on tissue microarray analysis as described above. Negative; +: weak positive; ++: positive; +++: strong positive. Weak positive and positive samples were considered as low OLFM4 level, strong positive samples were considered as high OLFM4 level. Scale bars, 100 μm. e Pie chart showing OLFM4 expression status in cardia and non-cardia gastric tumors respectively. f Immunoblotting showing the OLFM4 levels in cardia and non-cardia gastric tumors. g Bar plots showing the proportion of strong-positive OLFM4 in different EBV infectious samples. 153 samples are verified by both EBV FISH and TMA analysis. h Correlation of OLFM4 expression with EBV infection by performing Chi-Squared test. i Immunoblotting showing the expression of OLFM4 and EBNA-1 in GC tumor samples. j Diagram showing the anatomic location distribution of EBV infection and OLFM4 high expression. All lanes are loaded with 50 μg of total protein (f, i). Representative of two independent experiments (f, i).