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(A) Intrinsic fluorescence of α-LA, at pH 7.4 (
), and pH 2.0 (
). (B) Intrinsic Trp fluorescence emission spectra (λex = 295 nm) of α-LA (0.2 mg mL−1) without SHMP (
), and in the presence of different concentrations of 0.01 (
), 0.03 (
), 0.05 (
), 0.1 (
), 0.2 (
), 0.4 (
), 0.8 (
), 1.0 (
), 2.0 (
), and 5.0 (
) mM SHMP at pH 2.0. (C) The wavelength maximum was plotted against SHMP in mM concentration at pH 2.0.
