Fig. 3.

Dual-silencing efficacy of siRNA-conjugated SWNT delivery system. Fluorescence microscopic images of co-expressed GFP and RFP in H1299 cells incubated with 100 nM of (A) SWNT-PL-PEG-S-S-siRNA or (B) SWNT-PL-PEG-siRNA conjugates targeting GFP (siGFP) or RFP (siRFP) or LUC (siLUC; negative control) alone, or both (dual targeting; siGFP + siRFP) for 72 h. The cell nuclei were stained with DAPI. The furthest right panel was the combined images. Microscopic observation was captured under 100x magnification.