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. 2024 Dec 3;14:30098. doi: 10.1038/s41598-024-80646-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 4 — Energy dependent cellular internalization mechanisms of SWNT-siRNA conjugates independent of ABCB1. Graph shows the mean relative luminescent units (RLU) of (A and B) luciferase expressing H1299 cells co-treated with SWNT only (without siLUC), SWNT-PL-PEG-S-S-siLUC or SWNT-PL-PEG-siLUC with or without sub-lethal concentrations of various endocytosis inhibitors. NaN₃, chlorpromazine, genistein and m-βCD are inhibitors for energy-dependent cellular uptake, clathrin-mediated endocytosis, caveolae-dependent endocytosis and macro-pinocytosis, respectively. Bars represent mean ± SD of at least 3 independent experiments. * represents statistical significance as compared to SWNT-siLUC (Student’s t-test, p < 0.01). (C) Western blot of ABCB1 and loading control GAPDH, cropped blots. Ectopic expression of ABCB1 (multiple drug resistance) protein in H1299-LUC cells was confirmed by the immunoblotting with increased expression of ABCB1 at 141 kDA, while GAPDH at 36 kDA served as the loading control. Full blots available in Supplementary Figure S2-S3. (D) Expression of ABCB1 did not affect the delivery of siRNA by the SWNT. Bars represent mean ± SD of at least 3 independent experiments. * represents statistical significance as compared to controls (Student’s t-test, p < 0.01).