Fig. 6.

In vitro alkaline phosphatase (ALP) activity and osteogenic mineralization ability of porous scaffolds. A and B: rBMSCs were cultured in media prepared with extracts from four types of porous scaffolds, with ALP staining performed after 7 and 14 days of osteogenic induction (scale bars = 1 mm). C and D: The mineralized nodules were stained using Alizarin Red S dye after 14 and 21 days of osteogenic induction (scale bars = 1 mm). E: Quantitative ALP activity detection; F: Quantitative detection of mineralized nodules. All statistical data are represented as mean ± SD (n = 3; ∗P < 0.05, ∗∗∗∗P < 0.0001).