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In vitro osteogenic efficiency-related gene expression detection of porous scaffolds. A–D: rBMSCs were cocultured with four types of porous scaffolds. After 7 and 14 days of osteogenic induction, RT‒PCR was performed to detect the expression of osteogenic-related genes, including OPN (A), RunX2 (B), Col-1 (C), and OCN (D). E and F: Western blotting assay used to detect osteogenic-related proteins. All statistical data are represented as mean ± SD (n = 3; ∗∗∗∗P < 0.0001).
