Demarcation of a standard total mesorectal excision specimen on (A,
B) surgical and whole-mount histology (WMH) slices and
(C) radiology-pathology workflow of segmentation and
landmark definition. (A) Circumferential margins of the
rectal cancer resection specimen were marked with different ink colors
to distinguish anterior and posterior and right and left regions of the
specimen and enable proper orientation. (B) WMH slice of
the total mesorectal excision specimen demonstrates the color code that
was used to guide the spatial localization of the rectum portions, as
follows: black = anterior, red = posterior, blue = left, and green =
right. (C) A gastrointestinal pathologist and a radiologist
conducted a collaborative review of WMH and MR images to ensure precise
correspondence between pathology and high-resolution T2-weighted
imaging. Three corresponding levels were established for each patient at
WMH and MRI: the midpoint of the tumor bed, one slice or section above,
and one slice or section below. Subsequently, both experts manually
delineated the external rectal contour (the outer edge of the muscularis
propria), internal rectal contour (inner aspect of the mucosa), and
tumor bed at each designated level (illustrated at the midpoint level).
Additionally, the radiologist and pathologist annotated eight
corresponding point-based landmarks in each modality along the internal
and external borders of the rectal wall, encompassing the anterior,
posterior, leftward, and rightward directions.