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. 2024 Oct 25;6(6):e240073. doi: 10.1148/rycan.240073

Figure 3:

Coregistration workflow for the proposed linearized iterative boundary reconstruction (LIBR) MRI-histopathology fusion method after segmentation and landmark characterization as demonstrated in Figure 2. Prior to coregistration, eight point-based landmarks are annotated on the external and internal rectal contours on each MR and whole-mount histopathology (WMH) image. A series of regularized Kelvinlet control points is then distributed across the external and internal rectal contours, from which a biomechanical deformation basis is constructed. After point-based registration of landmarks, deformation of the histopathology sample relative to MRI is computed from the rectal wall contours via the LIBR approach. A series of control points are distributed across the external and internal rectal contours to establish a regularized Kelvinlet deformation basis for the WMH image. The LIBR approach estimates the deformation between WMH and MRI by maximizing the agreement between rectal wall contours subject to this deformation basis. Finally, the registered WMH is fused with MRI to indicate spatial correspondence between reference standard histopathologic features and MRI.

Coregistration workflow for the proposed linearized iterative boundary reconstruction (LIBR) MRI-histopathology fusion method after segmentation and landmark characterization as demonstrated in Figure 2. Prior to coregistration, eight point-based landmarks are annotated on the external and internal rectal contours on each MR and whole-mount histopathology (WMH) image. A series of regularized Kelvinlet control points is then distributed across the external and internal rectal contours, from which a biomechanical deformation basis is constructed. After point-based registration of landmarks, deformation of the histopathology sample relative to MRI is computed from the rectal wall contours via the LIBR approach. A series of control points are distributed across the external and internal rectal contours to establish a regularized Kelvinlet deformation basis for the WMH image. The LIBR approach estimates the deformation between WMH and MRI by maximizing the agreement between rectal wall contours subject to this deformation basis. Finally, the registered WMH is fused with MRI to indicate spatial correspondence between reference standard histopathologic features and MRI.