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. 2024 Jul 27;19:104–116. doi: 10.1016/j.aninu.2024.06.006

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© 2024 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2 — Coated sodium butyrate (CSB) ameliorated impairment of intestinal epithelial cell junctions induced by high-energy and low-protein (HELP) diets in laying hens. (A) mRNA relative expression of tight junction proteins of jejunum, n = 6. (B) Representative immunofluorescence images of β-Catenin (red), ZO-1 (red) and occludin (red) staining in the jejunum (magnification, 400×). CON = normal diet; HELP = HELP diet; CH500 = 500 mg/kg CSB added to HELP diet; MUC2 = mucin 2; ZO-1 = zonula occluden-1. ∗P < 0.05, ∗∗P < 0.01.