Figure 4.

Profiling of BM-derived CD8⁺ T cells of patients with HR-MDS and secondary AML with scRNA-seq. A, UMAP of CD8⁺ T cells identified 11 clusters. A total of 28,449 CD8⁺ T cells were pooled from four patients with HR-MDS (15,597 cells) and five patients with secondary AML (12,852 cells). B, Bubble plot depicting the average expression of genes used to characterize the clusters. C, Expression of selected genes projected onto UMAPs. D, Heatmap showing selected top DEGs for each cell cluster. E, Ridgeline plots displaying the cytotoxic signature score for each cell cluster, as defined by the expression of key-related genes. F, Ridgeline plots displaying the cell-cycle signature score for each cell cluster.