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. 2024 Jul 23;13(30):2401511. doi: 10.1002/adhm.202401511

Figure 2.

Figure 2

Generation of mature cholangiocytes from human intrahepatic cholangiocyte organoids (ICOs) in different hydrogels. A) Morphology of the mature cholangiocyte organoids in different hydrogels at four time points of differentiation (day 2, 4, 6, and 8). M+C, Matrigel/collagen type I mixed hydrogel; PIC+C, PIC/collagen I mixed hydrogel; PIC, PIC hydrogel. Scale bar = 500 µm. B) Gene expression analysis for cholangiocyte organoids in CDM conditions in different hydrogels after differentiation. Four independent donors in CDM conditions in three different hydrogels (M+C, PIC+C, and PIC hydrogel) at day 7. Results are shown as fold change relative to human gallbladder. Data are shown as box and whisker plots. Center line, median; box, interquartile; whiskers: minimum to maximum, shows all points. Statistical differences between the groups were tested using one‐way ANOVA followed by Dunn's test for multiple comparisons; n = 4, *p < 0.05.