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. 2024 Jul 23;13(30):2401511. doi: 10.1002/adhm.202401511

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© 2024 The Author(s). Advanced Healthcare Materials published by Wiley‐VCH GmbH

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Immunofluorescence analysis of the key cholangiocyte markers in organoids cultured in three different hydrogels (M+C, Matrigel/collagen I; PIC+C, PIC/collagen I; PIC). A) Cytoskeleton, epithelial and junction markers [keratin (K) 19, cadherin 1 (CDH1) and tight junction protein 1 (ZO1)]. B–E) Regulator of bile acid markers [secretin receptor (SCTR), G protein‐coupled bile acid receptor 1 (GPBAR1, also known as TGR5), Na⁺‐taurocholate cotransporting polypeptide (NTCP), multidrug resistance protein 3 (MRP3)]. (E) Transcription factor marker [SRY‐box transcription factor (SOX9)] and the water channel marker (F) aquaporin 1 (AQP1). Scale bar = 50 µm.