Figure 5.

Functional FXR signaling in cholangiocyte differentiated organoids in three different hydrogels (M+C, Matrigel/collagen I; PIC+C, PIC/collagen I; PIC). Gene expression analysis showing the activation of FXR signaling by downstream upregulated genes, SLC51A, SLC51B, downregulated genes SLC10A2 in DMSO (Control), FXR agonist (GW4064) and cholestatic condition (BA 100×) group. Data are shown as mean ± SD of four independent donors for each group. Statistical differences between the groups were tested using two‐way ANOVA; n = 4, *p < 0.05, **p < 0.01, ****p < 0.0001.