Fig. 7.

Specific effects of iMWA and Gr-1 blocking combined therapy in HCC mouse model. A Schematic diagram of treatment of experimental mice. Mice were injected subcutaneously with H22 cells and treated with sham operation (Ctrl) or iMWA (~ 50% ablation) after 2 weeks. After ablation, they received anti-IgG or Gr-1 treatment once a week and were executed at week 4. B Representative images of tumors from nude mice in two groups. C, D Tumor growth curves and tumor weights of each group. E Immunofluorescence of CD11b and Gr-1 in two groups, Scale bar, 20 μm. F Immunohistochemistry of CD8 in two groups, Scale bar, 20 μm. G The expression level of Ki67 in nude mice-derived xenograft tumor was determined by Immunofluorescence, Scale bar, 20 μm. *P < 0.05; **P < 0.01; ***P < 0.001