Table 2. Oligonucleotides used for the multiplex PCR protocols based on ARTIC-like strategy used in study of feline panleukopenia virus in wild animals, Abruzzo and Molise, Italy, January 2022–May 2023* .
| Primer | Sequence, 5′ → 3′ | Amplicon size, bp |
|---|---|---|
| CPV1_left | AATGATAGGCGGTTTGTGTGTTT | 396 |
| CPV1_right | CAACTTCCGATTCCCAGTCCAT | 396 |
| CPV2_left | CCAATTCAAAATGAAGAGCTAACATCTT | 410 |
| CPV2_right | GTCACCCATTCACTATCTTCTGCA | 410 |
| CPV3_left | TGGAGTAGATGGTTGGTGACTCT | 416 |
| CPV3_right | ACCAAGTCCCGCAAAGTACATT | 416 |
| CPV4_left | AGCACACTTTACACTGAACAAATGA | 401 |
| CPV4_right | GCTATAGCGTGACAAACTTTAATCCA | 401 |
| CPV5_left | AGCAAGAACAAAAACAGCATTTGAA | 402 |
| CPV5_right | TGATCAATTCTAATTGTTTGTCCAGAACA | 402 |
| CPV6_left | AAAAATTTAATTTGGATTGAAGAAGCTGGT | 407 |
| CPV6_right | CCTTCTTGTATTTTAGGCTCCGC | 407 |
| CPV7_left | TGAATCAACCATGGCTAACTATACACA | 393 |
| CPV7_right | GGAGGTGCCATCGTACCTTAATC | 393 |
| CPV8_left | TGGTCCGAAATAGAGGCAGACC | 393 |
| CPV8_right | CCCCCAATCTTTAGCGTCCTTA | 393 |
| CPV9_left | GCTGCTTATCTTCGCTCTGGTA | 412 |
| CPV9_right | AAATCCCCACACCCCCAGAA | 412 |
| CPV10_left | GCACCAATGAGTGATGGAGCA | 410 |
| CPV10_right | TCACTCATAGTATTAACAATTAGTTGCCA | 410 |
| CPV11_left | TGCACAAATTGTAACACCTTGGT | 388 |
| CPV11_right | ATTTGTTGGTGTGCCACTAGTTC | 388 |
| CPV12_left | ACCAACCATACCAACTCCATGG | 411 |
| CPV12_right | TAACCAACCTCAGCTGGTCTCA | 411 |
| CPV13_left | GGAGTTCAACAAGATAAAAGACGTGG | 396 |
| CPV13_right | ACCTCCAATTGGATCTGTTGGT | 396 |
| CPV14_left | TCCAGAAGGAGATTGGATTCAAAATATT | 411 |
| CPV14_right | TTCCAAGTATGAGAGGCTCTTAGTT | 411 |
| CPV15_left | TTGATACTGACTTAAAACCAAGACTTCA | 511 |
| CPV15_right | ACAGTTATTGTATACCATATAACAAACCTTCT | 511 |
*ARTIC-like strategy as shown in (5). CPV, canine parvovirus.